論文 - 藤井 穂高
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Identification of telomere-associated molecules by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP).
Fujita T, Asano Y, Ohtsuka J, Takada Y, Saito K, Ohki R, Fujii H
Scientific Reports 3 3171 2013年11月
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Efficient isolation of specific genomic regions and identification of associated proteins by engineered DNA-binding molecule-mediated chromatin immunoprecipitation (enChIP) using CRISPR.
Fujita T, Fujii H
Biochemical and Biophysical Research Communications 439 ( 1 ) 132 - 136 2013年09月
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Direct identification of insulator components by insertional chromatin immunoprecipitation.
Fujita T, Fujii H
PLoS One 6 ( 10 ) e26109 2011年10月
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Insertional chromatin immunoprecipitation: a method for isolating specific genomic regions.
Hoshino A, Fujii H
Journal of Bioscience and Bioengineering 108 ( 5 ) 446 - 449 2009年11月
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Regulation of cell proliferation by interleukin-3-induced nuclear translocation of pyruvate kinase.
Hoshino A, Hirst JA, Fujii H
The Journal of Biological Chemistry 282 ( 24 ) 17706 - 17711 2007年06月
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Oligoribonucleotide interference-PCR-based methods for the sensitive and accurate detection of KRAS mutations
Fujita H, Fujita T, Ishido K, Hakamada K, Fujii H
Biology Methods & Protocols 9 ( 1 ) bpae071 2024年10月
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Highly sensitive and accurate detection of ALK-TKI resistance mutations by oligoribonucleotide interference-PCR (ORNi-PCR)-based methods
Tabe C, Fujita T, Taima K, Tanaka H, Makiguchi T, Itoga M, Ishioka Y, Tasaka S, Fujii H
Lung Cancer 197 107969 2024年09月
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iChIP-SILAC analysis identifies epigenetic regulators of CpG methylation of the p16INK4A gene
Fujita T, Fujii H
FEBS Letters 2024年04月
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Moderate transgenic expression of CBR1 is not sufficient to prolong life expectancy in an SV40 T-antigen-induced gynecological carcinogenesis mouse model
Kadonosawa Y, Yokoyama M, Tatara Y, Miyoshi I, Araki Y, Yokoyama Y, Fujii H
弘前医学 74 99 - 107 2024年03月
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Real-time MBDi-RPA using methyl-CpG binding protein 2: A real-time detection method for simple and rapid estimation of CpG methylation status
Ishidoya M, Fujita T, Tasaka S, Fujii H
Analytica Chimica Acta 1302 342486 2024年03月
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GARP on hepatic stellate cells is essential for the development of liver fibrosis
Zhang X, Sharma P, Maschmeyer P, Hu Y, Lou M, Kim J, Fujii H, Unutmaz D, Schwabe RF, Winau F
Journal of Hepatology 79 ( 5 ) 1214 - 1225 2023年11月
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Oligoribonucleotide-mediated blockade of DNA extension by Taq DNA polymerases Increases specificity and sensitivity for detecting single-nucleotide differences
Fujita T, Nagata S, Fujii H
Analytical Chemistry 2023年02月
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Development of transgenic mice overexpressing mouse carbonyl reductase 1
Yokoyama M, Fujita T, Kadonosawa Y, Tatara Y, Motooka D, Ikawa M, Fujii H, Yokoayama Y
Molecular Biology Reports 2022年11月
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An enChIP system for the analysis of genome functions in budding yeast
Fujii H, Fujita T
Biology Methods & Protocols 7 ( 1 ) bpac025 2022年10月
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Circular RNAs hsa_circ_0001438 and hsa_circ_0000417 are downregulated and upregulated, respectively, in hepatocellular carcinoma
103 ( 6 ) 245 - 251 2022年09月
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IL-3-induced immediate expression of c-fos and c-jun is modulated by the IKK2-JNK axis
11 ( 9 ) 1451 2022年04月
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enChIP-Seq analyzer: a software program to analyze and interpret enChIP-Seq data for the detection of physical interactions between genomic regions
Sarudate A, Fujita T, Nakayama T, Fujii H
Genes 13 472 2022年03月
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A stem cell marker KLF5 regulates CCAT1 via three-dimensional genome structure in colorectal cancer cells.
Takeda T, Yokoyama Y, Takahashi H, Okuzaki D, Asai K, Itakura H, Miyoshi N, Kobayashi S, Uemura M, Fujita T, Ueno H, Mori M, Doki Y, Fujii H, Eguchi H, Yamamoto H
British Journal of Cancer 126 109 - 119 2021年10月
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Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations.
Fujita T, Nagata S, Fujii H
Communications Biology 4 ( 1 ) 988 - 988 2021年08月
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Corrigendum to "Cell type-specific roles of Jak3 in IL-2-induced proliferative signal transduction" [Biochem. Biophys. Res. Commun. 354 (3) (2007) 825-829].
Fujii H
Biochemical and Biophysical Research Communications 552 196 2021年05月
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Sequence-specific inhibition of reverse transcription by recombinant CRISPR/dCas13a ribonucleoprotein complexes <i>in vitro</i>.
Fujita T, Nagata S, Yuno M, Fujii H
Biology Methods & Protocols 6 ( 1 ) bpab009 2021年01月
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MSCV-based retroviral plasmids expressing 3xFLAG-Sp-dCas9 for enChIP analysis.
Yuno M, Nagata S, Fujita T, Fujii H
Biology Methods & Protocols 6 ( 1 ) bpab013 2021年
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pSIR-bsr, a self-inactivating retrovirus vector expressing the blasticidin S-resistance gene.
Fujii H
Biology Methods & Protocols 6 ( 1 ) bpab022 2021年
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SAMHD1-mediated dNTP degradation is required for efficient DNA repair during antibody class switch recombination.
Afzal Husain, Jianliang Xu, Hodaka Fujii, Mikiyo Nakata, Maki Kobayashi, Ji-Yang Wang, Jan Rehwinkel, Tasuku Honjo, Nasim A Begum
EMBO Journal 39 ( 15 ) e102931 - e102931 2020年08月
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Discrimination of CpG Methylation Status and Nucleotide Differences in Tissue Specimen DNA by Oligoribonucleotide Interference-PCR.
Takeshi Shimizu, Toshitsugu Fujita, Sakie Fukushi, Yuri Horino, Hodaka Fujii
International Journal of Molecular Sciences 21 ( 14 ) 2020年07月
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A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by T reg cells
Rabab Nasrallah, Charlotte J Imianowski, Lara Bossini-Castillo, Francis M Grant, Mikail Dogan, Lindsey Placek, Lina Kozhaya, Paula Kuo, Firas Sadiyah, Sarah K Whiteside, Maxwell R Mumbach, Dafni Glinos, Panagiota Vardaka, Carly E Whyte, Teresa Lozano, Toshitsugu Fujita, Hodaka Fujii, Adrian Liston, Simon Andrews, Adeline Cozzani, Jie Yang, Suman Mitra, Enrico Lugli, Howard Y Chang, Derya Unutmaz, Gosia Trynka, Rahul Roychoudhuri
Nature 583 ( 7816 ) 447 - 452 2020年06月
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Simultaneous detection of the T790M and L858R mutations in the EGFR Gene by oligoribonucleotide interference-PCR.
Keisuke Baba, Toshitsugu Fujita, Sadatomo Tasaka, Hodaka Fujii
International Journal of Molecular Sciences 20 ( 16 ) 4020 2019年08月
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Target enrichment from a DNA mixture by oligoribonucleotide interference-PCR (ORNi-PCR)
Toshitsugu Fujita, Daisuke Motooka, Hodaka Fujii
Biology Methods & Protocols 4 ( 1 ) bpz009 2019年08月
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Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice.
Fusako Kitaura, Miyuki Yuno, Toshitsugu Fujita, Shigeharu Wakana, Jun Ueda, Kazuo Yamagata, Hodaka Fujii
PLoS One 14 ( 7 ) e0220199 - e0220199 2019年07月
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A refined two-step oligoribonucleotide interference-PCR method for precise discrimination of nucleotide differences.
Toshitsugu Fujita, Miyuki Yuno, Fusako Kitaura, Hodaka Fujii
Scientific Reports 8 ( 1 ) 17195 - 17195 2018年11月
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Detection of genome-edited cells by oligoribonucleotide interference-PCR.
Toshitsugu Fujita, Miyuki Yuno, Fusako Kitaura, Hodaka Fujii
DNA Research 25 ( 4 ) 395 - 407 2018年08月
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An enChIP system for the analysis of bacterial genome functions.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
BMC Research Notes 11 ( 1 ) 387 - 387 2018年06月
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Promoter-associated proteins of EPAS1 identified by enChIP-MS - A putative role of HDX as a negative regulator.
Arash Hamidian, Marica Vaapil, Kristoffer von Stedingk, Toshitsugu Fujita, Camilla U Persson, Pontus Eriksson, Srinivas Veerla, Katleen De Preter, Frank Speleman, Hodaka Fujii, Sven Påhlman, Sofie Mohlin
Biochemical and Biophysical Research Communications 499 ( 2 ) 291 - 298 2018年05月
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enChIP systems using different CRISPR orthologues and epitope tags.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
BMC Research Notes 11 ( 1 ) 154 - 154 2018年02月
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Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis.
Toshitsugu Fujita, Fusako Kitaura, Asami Oji, Naoki Tanigawa, Miyuki Yuno, Masahito Ikawa, Ichiro Taniuchi, Hodaka Fujii
Genes to Cells 23 ( 4 ) 318 - 325 2018年02月
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Locus-specific ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken B cell line.
Toshitsugu Fujita, Fusako Kitaura, Miyuki Yuno, Yutaka Suzuki, Sumio Sugano, Hodaka Fujii
DNA Research 24 ( 5 ) 537 - 548 2017年10月
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Identification of physical interactions between genomic regions by enChIP-Seq.
Toshitsugu Fujita, Miyuki Yuno, Yutaka Suzuki, Sumio Sugano, Hodaka Fujii
Genes to Cells 22 ( 6 ) 506 - 520 2017年06月
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The role of platelet and endothelial GARP in thrombosis and hemostasis.
Elien Vermeersch, Frederik Denorme, Wim Maes, Simon F De Meyer, Karen Vanhoorelbeke, Justin Edwards, Ethan M Shevach, Derya Unutmaz, Hodaka Fujii, Hans Deckmyn, Claudia Tersteeg
PLoS One 12 ( 3 ) e0173329 - e0173329 2017年03月
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Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
Scientific Reports 6 30485 - 30485 2016年07月
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Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
Genes to Cells 21 ( 4 ) 370 - 377 2016年02月
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Identification of non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing.
Toshitsugu Fujita, Miyuki Yuno, Daisuke Okuzaki, Rieko Ohki, Hodaka Fujii
PLoS One 10 ( 4 ) e0123387 - e0123387 2015年04月
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A critical role of the Thy28-MYH9 axis in B cell-specific expression of the Pax5 gene in chicken B cells.
Fujita T, Kitaura F, Fujii H
PLoS One 10 ( 1 ) e0116579 2015年01月
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Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins.
Fujita T, Fujii H
BMC Molecular Biology 15 26 2014年11月
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Oligoribonucleotide (ORN) interference-PCR (ORNi-PCR): a simple method for suppressing PCR amplification of specific DNA sequences using ORNs.
Tanigawa N, Fujita T, Fujii H
PLoS One 9 ( 11 ) e113345 2014年11月
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Identification of proteins associated with an IFNγ-responsive promoter by a retroviral expression system for enChIP using CRISPR.
Fujita T, Fujii H
PLoS One 9 ( 7 ) e103084 2014年07月
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Regulation of the expression of GARP/latent TGF-β1 complexes on mouse T cells and their role in regulatory T cell and Th17 differentiation.
Edwards JP, Fujii H, Zhou AX, Creemers J, Unutmaz D, Shevach EM
Journal of Immunology 190 ( 11 ) 5506 - 5515 2013年06月
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GARP-TGF-β complexes negatively regulate regulatory T cell development and maintenance of peripheral CD4+ T cells in vivo
Angela X Zhou , Lina Kozhaya, Hodaka Fujii, Derya Unutmaz
Journal of Immunology 190 ( 10 ) 5057 - 5064 2013年05月
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Discovery and characterization of new transcripts from RNA-seq data in mouse CD4(+) T cells.
Hutchins AP, Poulain S, Fujii H, Miranda-Saavedra D
Genomics 100 ( 5 ) 303 - 313 2012年11月
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Transcription start sites and usage of the first exon of mouse Foxp3 gene.
Fujita T, Fujii H
Molecular Biology Reports 39 ( 10 ) 9613 - 9619 2012年10月
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Apurinic/apyrimidinic endonuclease1/redox factor-1 (Ape1/Ref-1) is essential for IL-21-induced signal transduction through ERK1/2 pathway.
Juliana FM, Nara H, Onoda T, Rahman M, Araki A, Jin L, Fujii H, Tanaka N, Hoshino T, Asao H
Biochemical and Biophysical Research Communications 420 ( 3 ) 628 - 634 2012年04月
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Species-specific 5'-genomic structure and multiple transcription start sites in the chicken Pax5 gene.
Fujita T, Fujii H
Gene 477 ( 1-2 ) 24 - 31 2011年05月
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Novel reporter cell line to analyze cytokine-mediated expression regulation of c-myc gene.
Fujii H
Journal of Bioscience and Bioengineering 108 ( 5 ) 438 - 440 2009年11月
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Expression of GARP selectively identifies activated human FOXP3+ regulatory T cells.
Wang R, Kozhaya L, Mercer F, Khaitan A, Fujii H, Unutmaz D
Proceedings of the National Academy of Sciences of the United States of America 106 ( 32 ) 13439 - 13444 2009年08月
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Regulation of Fas-mediated immune homeostasis by an activation-induced protein, Cyclon.
Saint Fleur S, Hoshino A, Kondo K, Egawa T, Fujii H
Blood 114 ( 7 ) 1355 - 1365 2009年08月
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Lack of nuclear translocation of cytoplasmic domains of IL-2/IL-15 receptor subunits.
Fujii H, Hoshino A
Cytokine 46 ( 3 ) 302 - 308 2009年06月
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Identification of a regulatory T cell specific cell surface molecule that mediates suppressive signals and induces Foxp3 expression.
Wang R, Wan Q, Kozhaya L, Fujii H, Unutmaz D
PLoS One 3 ( 7 ) e2705 2008年07月
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Receptor expression is essential for proliferation induced by dimerized Jak kinases.
Fujii H
Biochemical and Biophysical Research Communications 370 ( 4 ) 557 - 560 2008年06月
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Plasmodium circumsporozoite protein promotes the development of the liver stages of the parasite.
Singh AP, Buscaglia CA, Wang Q, Levay A, Nussenzweig DR, Walker JR, Winzeler EA, Fujii H, Fontoura BM, Nussenzweig V
Cell 131 ( 3 ) 492 - 504 2007年11月
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Temporal regulation of Stat5 activity in determination of cell differentiation program.
Hoshino A, Fujii H
Biochemical and Biophysical Research Communications 358 ( 3 ) 914 - 919 2007年07月
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Cell type-specific roles of Jak3 in IL-2-induced proliferative signal transduction.
Fujii H
Biochemical and Biophysical Research Communications 354 ( 3 ) 825 - 829 2007年03月
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Redundant promoter elements mediate IL-3-induced expression of a novel cytokine-inducible gene, cyclon.
Hoshino A, Fujii H
FEBS Letters 581 ( 5 ) 975 - 980 2007年03月
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Nuclear translocation of 2-amino-3-ketobutyrate coenzyme A ligase by cold and osmotic stress
HOSHINO A, FUJII H
Cell Stress & Chaperones 12 ( 2 ) 186 - 191 2007年01月
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Regulation of Stat1 protein expression by phenylalanine 172 in the coiled-coil domain.
Hoshino A, Saint Fleur S, Fujii H
Biochemical and Biophysical Research Communications 346 ( 3 ) 1062 - 1066 2006年08月
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Inducible translocation trap: a system for detecting inducible nuclear translocation.
Hoshino A, Matsumura S, Kondo K, Hirst JA, Fujii H
Molecular Cell 15 ( 1 ) 153 - 159 2004年07月
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Enforced expression of the Ikaros isoform IK5 decreases the numbers of extrathymic intraepithelial lymphocytes and natural killer 1.1+ T cells.
Tucker SN, Jessup HK, Fujii H, Wilson CB
Blood 99 ( 2 ) 513 - 519 2002年01月
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Defective expression of the interleukin-2/interleukin-15 receptor beta subunit leads to a natural killer cell-deficient form of severe combined immunodeficiency
KC Gilmour, H Fujii, T Cranston, EG Davies, C Kinnon, HB Gaspar
BLOOD 98 ( 3 ) 877 - 879 2001年08月
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Differential requirement of the cytoplasmic subregions of gamma c chain in T cell development and function
S Tsujino, JP Di Santo, A Takaoka, TL McKernan, S Noguchi, C Taya, H Yonekawa, T Saito, T Taniguchi, H Fujii
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 97 ( 19 ) 10514 - 10519 2000年09月
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Critical role of the membrane-proximal, proline-rich motif of the interleukin-2 receptor gamma c chain in the Jak3-independent signal transduction
S Tsujino, T Miyazaki, A Kawahara, M Maeda, T Taniguchi, H Fujii
GENES TO CELLS 4 ( 6 ) 363 - 373 1999年06月
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Protein tyrosine kinase Pyk2 mediates the Jak-dependent activation of MAPK and Stat1 in IFN-gamma, but not IFN-alpha, signaling
A Takaoka, N Tanaka, Y Mitani, T Miyazaki, H Fujii, M Sato, P Kovarik, T Decker, J Schlessinger, T Taniguchi
EMBO JOURNAL 18 ( 9 ) 2480 - 2488 1999年05月
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Functional dissection of the cytoplasmic subregions of the IL-2 receptor beta c chain in primary lymphocyte populations
H Fujii, K Ogasawara, H Otsuka, M Suzuki, K Yamamura, T Yokochi, T Miyazaki, H Suzuki, TW Mak, S Taki, T Taniguchi
EMBO JOURNAL 17 ( 22 ) 6551 - 6557 1998年11月
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Pyk2 is a downstream mediator of the IL-2 receptor-coupled Jak signaling pathway
T Miyazaki, A Takaoka, L Nogueira, Dikic, I, H Fujii, S Tsujino, Y Mitani, M Maeda, J Schlessinger, T Taniguchi
GENES & DEVELOPMENT 12 ( 6 ) 770 - 775 1998年03月
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ACTIVATION OF STAT5 BY INTERLEUKIN-2 REQUIRES A CARBOXYL-TERMINAL REGION OF THE INTERLEUKIN-2 RECEPTOR-BETA CHAIN BUT IS NOT ESSENTIAL FOR THE PROLIFERATIVE SIGNAL TRANSMISSION
H FUJII, Y NAKAGAWA, U SCHINDLER, A KAWAHARA, H MORI, F GOUILLEUX, B GRONER, JN IHLE, Y MINAMI, T MIYAZAKI, T TANIGUCHI
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 92 ( 12 ) 5482 - 5486 1995年06月
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FUNCTIONAL ACTIVATION OF JAK1 AND JAK3 BY SELECTIVE ASSOCIATION WITH IL-2 RECEPTOR SUBUNITS
T MIYAZAKI, A KAWAHARA, H FUJII, Y NAKAGAWA, Y MINAMI, ZJ LIU, OISHI, I, O SILVENNOINEN, BA WITTHUHN, JN IHLE, T TANIGUCHI
SCIENCE 266 ( 5187 ) 1045 - 1047 1994年11月
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REGULATION OF PROTEIN-KINASE-C ISOFORM PROTEINS IN PHORBOL ESTER-STIMULATED JURKAT T-LYMPHOMA CELLS
A TSUTSUMI, M KUBO, H FUJII, J FREIREMOAR, CW TURCK, JT RANSOM
JOURNAL OF IMMUNOLOGY 150 ( 5 ) 1746 - 1754 1993年03月