Affiliation
Graduate School of Medicine Medical Science Course Biochemistry and Genome Biology
FUJITA Toshitsugu
Degree
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博士（理学）
Research Interests
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Cell Biology
Research Areas
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Life Science / Applied biochemistry
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Life Science / Genome biology
Professional Memberships
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日本分子生物学会
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日本エピジェネティクス研究会
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THE JAPANESE SOCIETY FOR IMMUNOLOGY
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日本癌学会
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日本分子生物学会
Papers
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Real-time MBDi-RPA using methyl-CpG binding protein 2: A real-time detection method for simple and rapid estimation of CpG methylation status.
Ishidoya M, Fujita T, Tasaka S, Fujii H
Analytica Chimica Acta 2024
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iChIP-SILAC analysis identifies epigenetic regulators of CpG methylation of the p16INK4A gene
Fujita T, Fujii H
FEBS Letters 2024
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Oligoribonucleotide-Mediated Blockade of DNA Extension by Taq DNA Polymerases Increases Specificity and Sensitivity for Detecting Single-Nucleotide Differences.
Fujita T, Nagata S, Fujii H
Analytical chemistry 2023.2
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Development of transgenic mice overexpressing mouse carbonyl reductase 1.
Minako Yokoyama, Toshitsugu Fujita, Yuka Kadonosawa, Yota Tatara, Daisuke Motooka, Masahito Ikawa, Hodaka Fujii, Yoshihito Yokoayama
Molecular biology reports 50 ( 1 ) 531 - 540 2022.11
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An enChIP system for the analysis of genome functions in budding yeast.
Hodaka Fujii, Toshitsugu Fujita
Biology methods & protocols 7 ( 1 ) bpac025 2022.10
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Circular RNAs hsa_circ_0001438 and hsa_circ_0000417 are downregulated and upregulated, respectively, in hepatocellular carcinoma.
Sachiko Imanishi, Shoko Nagata, Toshitsugu Fujita, Hodaka Fujii
International journal of experimental pathology 103 ( 6 ) 245 - 251 2022.9
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Oligoribonucleotide interference-PCR: principles and applications.
Takeshi Shimizu, Toshitsugu Fujita, Hodaka Fujii
Biology methods & protocols 7 ( 1 ) bpac010 2022.5
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IL-3-Induced Immediate Expression of c-fos and c-jun Is Modulated by the IKK2-JNK Axis
Hirotaka Fujita, Toshitsugu Fujita, Hodaka Fujii
Cells 11 ( 9 ) 1451 - 1451 2022.4
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enChIP-Seq Analyzer: A Software Program to Analyze and Interpret enChIP-Seq Data for the Detection of Physical Interactions between Genomic Regions
13 472 2022.3
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A stem cell marker KLF5 regulates CCAT1 via three-dimensional genome structure in colorectal cancer cells.
1) Takeda, T., Yokoyama, Y., Takahashi, T., Okuzak,i D., Asai, K., Itakura, H., Miyoshi, N., Kobayashi, S., Uemura, M., Fujita, T., Ueno, H., Mori, M., Doki, Y., Fujii, H., Eguchi, H., Yamamoto, H.
British journal of cancer 126 ( 1 ) 109 - 119 2021.10
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Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations.
Fujita, T., Nagata, S., and Fujii, H.
Communications Biology 4 ( 1 ) 988 - 988 2021.8
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MSCV-based retroviral plasmids expressing 3xFLAG-Sp-dCas9 for enChIP analysis.
Miyuki Yuno, Shoko Nagata, Toshitsugu Fujita, Hodaka Fujii
Biology methods and protocols 6 ( 1 ) bpab013 2021.7
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Sequence-specific inhibition of reverse transcription by recombinant CRISPR/dCas13a ribonucleoprotein complexes in vitro.
Fujita, T., Nagata, S., and Fujii, H.
Biology methods and protocols 6 ( 1 ) bpab009 2021.4
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Locus-Specific Genomic DNA Purification Using the CRISPR System: Methods and Applications.
Hirotaka Fujita, Toshitsugu Fujita, Hodaka Fujii
The CRISPR journal 4 ( 2 ) 290 - 300 2021.4
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Discrimination of CpG Methylation Status and Nucleotide Dierences in Tissue Specimen DNA by Oligoribonucleotide Interference PCR
Takeshi Shimizu, Toshitsugu Fujita, Sakie Fukushi, Yuri Horino, Hodaka Fujii
International Journal of Molecular Sciences 21 5119 2020.7
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Discrimination of CpG Methylation Status and Nucleotide Differences in Tissue Specimen DNA by Oligoribonucleotide Interference-PCR.
Takeshi Shimizu, Toshitsugu Fujita, Sakie Fukushi, Yuri Horino, Hodaka Fujii
International journal of molecular sciences 21 ( 14 ) 2020.7
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A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by Treg cells
Rabab Nasrallah, Charlotte J. Imianowski, Lara Bossini-Castillo, Francis M. Grant, Mikail Dogan, Lindsey Placek, Lina Kozhaya, Paula Kuo, Firas Sadiyah, Sarah K. Whiteside, Maxwell R. Mumbach, Dafni Glinos, Panagiota Vardaka, Carly E. Whyte, Teresa Lozano, Toshitsugu Fujita, Hodaka Fujii, Adrian Liston, Simon Andrews, Adeline Cozzani, Jie Yang, Suman Mitra, Enrico Lugli, Howard Y. Chang, Derya Unutmaz, Gosia Trynka, Rahul Roychoudhuri
Nature 583 ( 7816 ) 447 - 452 2020.5
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＜一般演題抄録＞血液および固定組織を用いたORNi-PCRによる塩基配列差異の簡易診断法の開発
福士 咲恵, 清水 武史, 藤田 敏次, 藤井 穂高
弘前医学 71 ( 1 ) 97 - 97 2020
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Simultaneous Detection of the T790M and L858R Mutations in the EGFR Gene by Oligoribonucleotide Interference-PCR.
Keisuke Baba, Toshitsugu Fujita, Sadatomo Tasaka, Hodaka Fujii
International Journal of Molecular Sciences 20 ( 16 ) E4020 2019.8
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Target enrichment from a DNA mixture by oligoribonucleotide interference-PCR (ORNi-PCR).
Fujita, T, Motooka, D, Fujii, H
BIOLOGY Methods and Protocol 4 ( 1 ) bpz009 2019.8
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Simultaneous Detection of the T790M and L858R Mutations in the <i>EGFR</i> Gene by Oligoribonucleotide Interference-PCR.
Baba K, Fujita T, Tasaka S, Fujii H
International journal of molecular sciences 20 ( 16 ) 2019.8
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Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice.
Fusako Kitaura, Miyuki Yuno, Toshitsugu Fujita, Shigeharu Wakana, Jun Ueda, Kazuo Yamagata, Hodaka Fujii
PLoS One 14 ( 7 ) e0220199 2019.7
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Purification of specific DNA species using the CRISPR system
Fujita, T, Fujii, H
BIOLOGY Methods and Protocol 4 ( 1 ) bpz008 2019
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A refined two-step oligoribonucleotide interference-PCR method for precise discrimination of nucleotide differences.
Toshitsugu Fujita, Miyuki Yuno, Fusako Kitaura, Hodaka Fujii
Scientific Reports 8 ( 1 ) 17195 2018.11
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Detection of genome-edited cells by oligoribonucleotide interference-PCR.
Toshitsugu Fujita, Miyuki Yuno, Fusako Kitaura, Hodaka Fujii
DNA Research 25 ( 4 ) 395 - 407 2018.8
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An enChIP system for the analysis of bacterial genome functions.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
BMC Research Notes 11 ( 1 ) 387 2018.6
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Promoter-associated proteins of EPAS1 identified by enChIP-MS - A putative role of HDX as a negative regulator.
Arash Hamidian, Marica Vaapil, Kristoffer von Stedingk, Toshitsugu Fujita, Camilla U. Persson, Pontus Eriksson, Srinivas Veerla, Katleen De Preter, Frank Speleman, Hodaka Fujii, Sven Påhlman, Sofie Mohlin
Biochemical and biophysical research communications 499 ( 2 ) 291 - 298 2018.5
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enChIP systems using different CRISPR orthologues and epitope tags.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
BMC research notes 11 ( 1 ) 154 - 154 2018.2
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Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis.
Toshitsugu Fujita, Fusako Kitaura, Asami Oji, Naoki Tanigawa, Miyuki Yuno, Masahito Ikawa, Ichiro Taniuchi, Hodaka Fujii
Genes to cells 2018.2
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Locus-specific ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken B cell line.
Toshitsugu Fujita, Fusako Kitaura, Miyuki Yuno, Yutaka Suzuki, Sumio Sugano, Hodaka Fujii
DNA research 24 ( 5 ) 537 - 548 2017.10
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Identification of physical interactions between genomic regions by enChIP-Seq.
Toshitsugu Fujita, Miyuki Yuno, Yutaka Suzuki, Sumio Sugano, Hodaka Fujii
Genes to cells 22 ( 6 ) 506 - 520 2017.6
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Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
Scientific reports 6 30485 - 30485 2016.7
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Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
Genes to cells 21 ( 4 ) 370 - 377 2016.4
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Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP.
Toshitsugu Fujita, Hodaka Fujii
Journal of visualized experiments : JoVE ( 107 ) e53478 - e53478 2016.1
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Identification of non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing.
Toshitsugu Fujita, Miyuki Yuno, Daisuke Okuzaki, Rieko Ohki, Hodaka Fujii
PloS one 10 ( 4 ) e0123387 - e0123387 2015.4
Books
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病理と臨床,40巻2号
藤田裕貴, 藤田敏次, 藤井穂高（ Role： Joint author , 156-161）
文光堂 2022.2
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実験医学別冊 完全版 ゲノム編集実験スタンダード
山本卓、佐久間哲史、他（ Role： Contributor）
羊土社 2019.12
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HANDBOOK OF EPIGENETICS (2nd edition)
Toshitsugu Fujita, Hodaka Fujii（ Role： Contributor）
Elsevier 2017.8
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生体の科学
藤井穂高、藤田敏次（ Role： Contributor）
医学書院 2017.6
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進化するゲノム編集
藤田敏次、藤井穂高（ Role： Contributor）
株式会社エヌ・ティー・エス 2015.10
Review Papers
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Locus-Specific Genomic DNA Purification Using the CRISPR System: Methods and Applications.
Fujita, H., Fujita, T. and Fujii, H.
CRISPR Journal 4 290 - 300 2021.4
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The regulation mechanism of KLF5 gene expression via three-dimensional genome structure in colorectal cancer
Yuhki Yokoyama, Takashi Takeda, Toshitsugu Fujita, Daisuke Okuzaki, Mami Sugiyama, Kaho Asai, Hidekazu Takahashi, Hodaka Fujii, Masaki Mori, Hirofumi Yamamoto
CANCER SCIENCE 112 338 - 338 2021.2
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Purification of specific DNA species using the CRISPR system
Toshitsugu Fujita, Hodaka Fujii
Biology Methods and Protocols 4 ( 1 ) bpz008 2019.7
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In vitro Engineered DNA-binding Molecule-mediated Chromatin Immunoprecipitation (in vitro enChIP) Using CRISPR Ribonucleoproteins in Combination with Next-generation Sequencing (in vitro enChIP-Seq) for the Identification of Chromosomal Interactions
Toshitsugu Fujita, Hodaka Fujii
Bio-protocol 7 ( 22 ) e2612 - e2612 2017.11
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Biochemical Analysis of Genome Functions Using Locus-Specific Chromatin Immunoprecipitation Technologies
Toshitsugu Fujita, Hodaka Fujii
Gene regulation and systems biology 10 ( Suppl 1 ) 1 - 9 2016.1
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Applications of Engineered DNA-Binding Molecules Such as TAL Proteins and the CRISPR/Cas System in Biology Research
Toshitsugu Fujita, Hodaka Fujii
International journal of molecular sciences 16 ( 10 ) 23143 - 23164 2015.9
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Isolation of Specific Genomic Regions and Identification of Their Associated Molecules by Engineered DNA-Binding Molecule-Mediated Chromatin Immunoprecipitation (enChIP) Using the CRISPR System and TAL Proteins
Toshitsugu Fujita, Hodaka Fujii
International journal of molecular sciences 16 ( 9 ) 21802 - 21812 2015.9
Awards
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第26回弘前大学医学部学術賞（特別賞）
2022.01
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第5回バイオインダストリー奨励賞
2021.10
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平成30年度弘前大学学術特別賞（若手優秀論文賞）
2018.12
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平成28年度日本エピジェネティクス研究会 奨励賞
2016.05