論文 - 藤田 敏次
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Real-time MBDi-RPA using methyl-CpG binding protein 2: A real-time detection method for simple and rapid estimation of CpG methylation status.
Ishidoya M, Fujita T, Tasaka S, Fujii H
Analytica Chimica Acta 2024年
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iChIP-SILAC analysis identifies epigenetic regulators of CpG methylation of the p16INK4A gene
Fujita T, Fujii H
FEBS Letters 2024年
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Oligoribonucleotide-Mediated Blockade of DNA Extension by Taq DNA Polymerases Increases Specificity and Sensitivity for Detecting Single-Nucleotide Differences.
Fujita T, Nagata S, Fujii H
Analytical chemistry 2023年02月
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Development of transgenic mice overexpressing mouse carbonyl reductase 1.
Yokoyama M, Fujita T, Kadonosawa Y, Tatara Y, Motooka D, Ikawa M, Fujii H, Yokoayama Y
Molecular biology reports 50 ( 1 ) 531 - 540 2022年11月
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An enChIP system for the analysis of genome functions in budding yeast.
Fujii H, Fujita T
Biology methods & protocols 7 ( 1 ) bpac025 2022年10月
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Circular RNAs hsa_circ_0001438 and hsa_circ_0000417 are downregulated and upregulated, respectively, in hepatocellular carcinoma.
Imanishi S, Nagata S, Fujita T, Fujii H
International journal of experimental pathology 103 ( 6 ) 245 - 251 2022年09月
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Oligoribonucleotide interference-PCR: principles and applications.
Shimizu T, Fujita T, Fujii H
Biology methods & protocols 7 ( 1 ) bpac010 2022年05月
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IL-3-Induced Immediate Expression of c-fos and c-jun Is Modulated by the IKK2-JNK Axis.
Fujita H, Fujita T, Fujii H
Cells 11 ( 9 ) 1451 - 1451 2022年04月
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enChIP-Seq Analyzer: A Software Program to Analyze and Interpret enChIP-Seq Data for the Detection of Physical Interactions between Genomic Regions
13 472 2022年03月
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A stem cell marker KLF5 regulates CCAT1 via three-dimensional genome structure in colorectal cancer cells.
Takeda T, Yokoyama Y, Takahashi H, Okuzaki D, Asai K, Itakura H, Miyoshi N, Kobayashi S, Uemura M, Fujita T, Ueno H, Mori M, Doki Y, Fujii H, Eguchi H, Yamamoto H
British journal of cancer 126 ( 1 ) 109 - 119 2021年10月
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Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations.
Fujita T, Nagata S, Fujii H
Communications biology 4 ( 1 ) 988 - 988 2021年08月
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MSCV-based retroviral plasmids expressing 3xFLAG-Sp-dCas9 for enChIP analysis.
Yuno, M., Nagata, S., Fujita, T., and Fujii, H.
Biology methods & protocols 6 ( 1 ) bpab013 2021年07月
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Sequence-specific inhibition of reverse transcription by recombinant CRISPR/dCas13a ribonucleoprotein complexes in vitro.
Fujita T, Nagata S, Yuno M, Fujii H
Biology methods & protocols 6 ( 1 ) bpab009 2021年04月
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Locus-Specific Genomic DNA Purification Using the CRISPR System: Methods and Applications.
Fujita H, Fujita T, Fujii H
The CRISPR journal 4 ( 2 ) 290 - 300 2021年04月
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Discrimination of CpG Methylation Status and Nucleotide Dierences in Tissue Specimen DNA by Oligoribonucleotide Interference PCR
Takeshi Shimizu, Toshitsugu Fujita, Sakie Fukushi, Yuri Horino, Hodaka Fujii
International Journal of Molecular Sciences 21 5119 2020年07月
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Discrimination of CpG Methylation Status and Nucleotide Differences in Tissue Specimen DNA by Oligoribonucleotide Interference-PCR.
Shimizu T, Fujita T, Fukushi S, Horino Y, Fujii H
International journal of molecular sciences 21 ( 14 ) 2020年07月
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A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by Treg cells
Rabab Nasrallah, Charlotte J. Imianowski, Lara Bossini-Castillo, Francis M. Grant, Mikail Dogan, Lindsey Placek, Lina Kozhaya, Paula Kuo, Firas Sadiyah, Sarah K. Whiteside, Maxwell R. Mumbach, Dafni Glinos, Panagiota Vardaka, Carly E. Whyte, Teresa Lozano, Toshitsugu Fujita, Hodaka Fujii, Adrian Liston, Simon Andrews, Adeline Cozzani, Jie Yang, Suman Mitra, Enrico Lugli, Howard Y. Chang, Derya Unutmaz, Gosia Trynka, Rahul Roychoudhuri
Nature 583 ( 7816 ) 447 - 452 2020年05月
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＜一般演題抄録＞血液および固定組織を用いたORNi-PCRによる塩基配列差異の簡易診断法の開発
福士 咲恵, 清水 武史, 藤田 敏次, 藤井 穂高
弘前医学 71 ( 1 ) 97 - 97 2020年
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Simultaneous Detection of the T790M and L858R Mutations in the EGFR Gene by Oligoribonucleotide Interference-PCR.
Keisuke Baba, Toshitsugu Fujita, Sadatomo Tasaka, Hodaka Fujii
International Journal of Molecular Sciences 20 ( 16 ) E4020 2019年08月
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Target enrichment from a DNA mixture by oligoribonucleotide interference-PCR (ORNi-PCR)
Toshitsugu Fujita, Daisuke Motooka, Hodaka Fujii
Biology Methods and Protocols 4 ( 1 ) bpz009 2019年08月
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Simultaneous Detection of the T790M and L858R Mutations in the <i>EGFR</i> Gene by Oligoribonucleotide Interference-PCR.
Baba K, Fujita T, Tasaka S, Fujii H
International journal of molecular sciences 20 ( 16 ) 2019年08月
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Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice.
Fusako Kitaura, Miyuki Yuno, Toshitsugu Fujita, Shigeharu Wakana, Jun Ueda, Kazuo Yamagata, Hodaka Fujii
PLoS One 14 ( 7 ) e0220199 2019年07月
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Purification of specific DNA species using the CRISPR system
Fujita, T, Fujii, H
BIOLOGY Methods and Protocol 4 ( 1 ) bpz008 2019年
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A refined two-step oligoribonucleotide interference-PCR method for precise discrimination of nucleotide differences.
Toshitsugu Fujita, Miyuki Yuno, Fusako Kitaura, Hodaka Fujii
Scientific Reports 8 ( 1 ) 17195 2018年11月
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Detection of genome-edited cells by oligoribonucleotide interference-PCR.
Toshitsugu Fujita, Miyuki Yuno, Fusako Kitaura, Hodaka Fujii
DNA Research 25 ( 4 ) 395 - 407 2018年08月
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An enChIP system for the analysis of bacterial genome functions.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
BMC Research Notes 11 ( 1 ) 387 2018年06月
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Promoter-associated proteins of EPAS1 identified by enChIP-MS - A putative role of HDX as a negative regulator.
Arash Hamidian, Marica Vaapil, Kristoffer von Stedingk, Toshitsugu Fujita, Camilla U. Persson, Pontus Eriksson, Srinivas Veerla, Katleen De Preter, Frank Speleman, Hodaka Fujii, Sven Påhlman, Sofie Mohlin
Biochemical and biophysical research communications 499 ( 2 ) 291 - 298 2018年05月
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enChIP systems using different CRISPR orthologues and epitope tags.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
BMC research notes 11 ( 1 ) 154 - 154 2018年02月
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Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis.
Toshitsugu Fujita, Fusako Kitaura, Asami Oji, Naoki Tanigawa, Miyuki Yuno, Masahito Ikawa, Ichiro Taniuchi, Hodaka Fujii
Genes to cells 2018年02月
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Locus-specific ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken B cell line.
Toshitsugu Fujita, Fusako Kitaura, Miyuki Yuno, Yutaka Suzuki, Sumio Sugano, Hodaka Fujii
DNA research 24 ( 5 ) 537 - 548 2017年10月
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Identification of physical interactions between genomic regions by enChIP-Seq.
Toshitsugu Fujita, Miyuki Yuno, Yutaka Suzuki, Sumio Sugano, Hodaka Fujii
Genes to cells 22 ( 6 ) 506 - 520 2017年06月
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Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
Scientific reports 6 30485 - 30485 2016年07月
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Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
Genes to cells 21 ( 4 ) 370 - 377 2016年04月
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Isolation of Specific Genomic Regions and Identification of Associated Molecules by enChIP.
Toshitsugu Fujita, Hodaka Fujii
Journal of visualized experiments : JoVE ( 107 ) e53478 - e53478 2016年01月
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Identification of non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing.
Toshitsugu Fujita, Miyuki Yuno, Daisuke Okuzaki, Rieko Ohki, Hodaka Fujii
PloS one 10 ( 4 ) e0123387 - e0123387 2015年04月