論文 - 藤井 穂高
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pSIR-bsr, a self-inactivating retrovirus vector expressing the blasticidin S-resistance gene.
Fujii H
Biology Methods & Protocols 6 ( 1 ) bpab022 2021年
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SAMHD1-mediated dNTP degradation is required for efficient DNA repair during antibody class switch recombination.
Afzal Husain, Jianliang Xu, Hodaka Fujii, Mikiyo Nakata, Maki Kobayashi, Ji-Yang Wang, Jan Rehwinkel, Tasuku Honjo, Nasim A Begum
EMBO Journal 39 ( 15 ) e102931 - e102931 2020年08月
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Discrimination of CpG Methylation Status and Nucleotide Differences in Tissue Specimen DNA by Oligoribonucleotide Interference-PCR.
Takeshi Shimizu, Toshitsugu Fujita, Sakie Fukushi, Yuri Horino, Hodaka Fujii
International Journal of Molecular Sciences 21 ( 14 ) 2020年07月
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A distal enhancer at risk locus 11q13.5 promotes suppression of colitis by T reg cells
Rabab Nasrallah, Charlotte J Imianowski, Lara Bossini-Castillo, Francis M Grant, Mikail Dogan, Lindsey Placek, Lina Kozhaya, Paula Kuo, Firas Sadiyah, Sarah K Whiteside, Maxwell R Mumbach, Dafni Glinos, Panagiota Vardaka, Carly E Whyte, Teresa Lozano, Toshitsugu Fujita, Hodaka Fujii, Adrian Liston, Simon Andrews, Adeline Cozzani, Jie Yang, Suman Mitra, Enrico Lugli, Howard Y Chang, Derya Unutmaz, Gosia Trynka, Rahul Roychoudhuri
Nature 583 ( 7816 ) 447 - 452 2020年06月
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Simultaneous detection of the T790M and L858R mutations in the EGFR Gene by oligoribonucleotide interference-PCR.
Keisuke Baba, Toshitsugu Fujita, Sadatomo Tasaka, Hodaka Fujii
International Journal of Molecular Sciences 20 ( 16 ) 4020 2019年08月
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Target enrichment from a DNA mixture by oligoribonucleotide interference-PCR (ORNi-PCR)
Toshitsugu Fujita, Daisuke Motooka, Hodaka Fujii
Biology Methods & Protocols 4 ( 1 ) bpz009 2019年08月
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Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice.
Fusako Kitaura, Miyuki Yuno, Toshitsugu Fujita, Shigeharu Wakana, Jun Ueda, Kazuo Yamagata, Hodaka Fujii
PLoS One 14 ( 7 ) e0220199 - e0220199 2019年07月
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A refined two-step oligoribonucleotide interference-PCR method for precise discrimination of nucleotide differences.
Toshitsugu Fujita, Miyuki Yuno, Fusako Kitaura, Hodaka Fujii
Scientific Reports 8 ( 1 ) 17195 - 17195 2018年11月
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Detection of genome-edited cells by oligoribonucleotide interference-PCR.
Toshitsugu Fujita, Miyuki Yuno, Fusako Kitaura, Hodaka Fujii
DNA Research 25 ( 4 ) 395 - 407 2018年08月
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An enChIP system for the analysis of bacterial genome functions.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
BMC Research Notes 11 ( 1 ) 387 - 387 2018年06月
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Promoter-associated proteins of EPAS1 identified by enChIP-MS - A putative role of HDX as a negative regulator.
Arash Hamidian, Marica Vaapil, Kristoffer von Stedingk, Toshitsugu Fujita, Camilla U Persson, Pontus Eriksson, Srinivas Veerla, Katleen De Preter, Frank Speleman, Hodaka Fujii, Sven Påhlman, Sofie Mohlin
Biochemical and Biophysical Research Communications 499 ( 2 ) 291 - 298 2018年05月
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enChIP systems using different CRISPR orthologues and epitope tags.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
BMC Research Notes 11 ( 1 ) 154 - 154 2018年02月
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Transgenic mouse lines expressing the 3xFLAG-dCas9 protein for enChIP analysis.
Toshitsugu Fujita, Fusako Kitaura, Asami Oji, Naoki Tanigawa, Miyuki Yuno, Masahito Ikawa, Ichiro Taniuchi, Hodaka Fujii
Genes to Cells 23 ( 4 ) 318 - 325 2018年02月
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Locus-specific ChIP combined with NGS analysis reveals genomic regulatory regions that physically interact with the Pax5 promoter in a chicken B cell line.
Toshitsugu Fujita, Fusako Kitaura, Miyuki Yuno, Yutaka Suzuki, Sumio Sugano, Hodaka Fujii
DNA Research 24 ( 5 ) 537 - 548 2017年10月
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Identification of physical interactions between genomic regions by enChIP-Seq.
Toshitsugu Fujita, Miyuki Yuno, Yutaka Suzuki, Sumio Sugano, Hodaka Fujii
Genes to Cells 22 ( 6 ) 506 - 520 2017年06月
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The role of platelet and endothelial GARP in thrombosis and hemostasis.
Elien Vermeersch, Frederik Denorme, Wim Maes, Simon F De Meyer, Karen Vanhoorelbeke, Justin Edwards, Ethan M Shevach, Derya Unutmaz, Hodaka Fujii, Hans Deckmyn, Claudia Tersteeg
PLoS One 12 ( 3 ) e0173329 - e0173329 2017年03月
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Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
Scientific Reports 6 30485 - 30485 2016年07月
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Efficient sequence-specific isolation of DNA fragments and chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins.
Toshitsugu Fujita, Miyuki Yuno, Hodaka Fujii
Genes to Cells 21 ( 4 ) 370 - 377 2016年02月
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Identification of non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing.
Toshitsugu Fujita, Miyuki Yuno, Daisuke Okuzaki, Rieko Ohki, Hodaka Fujii
PLoS One 10 ( 4 ) e0123387 - e0123387 2015年04月
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A critical role of the Thy28-MYH9 axis in B cell-specific expression of the Pax5 gene in chicken B cells.
Fujita T, Kitaura F, Fujii H
PLoS One 10 ( 1 ) e0116579 2015年01月